Brain-derived Neurotrophic Factor in the Aqueous Humor of Glaucoma Patients
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- Igarashi Tsutomu
- Department of Ophthalmology, Nippon Medical School
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- Nakamoto Kenji
- Department of Ophthalmology, Nippon Medical School
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- Kobayashi Maika
- Department of Ophthalmology, Nippon Medical School
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- Suzuki Hisaharu
- Department of Ophthalmology, Nippon Medical School
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- Arima Takeshi
- Department of Ophthalmology, Nippon Medical School
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- Tobita Yutaro
- Department of Ophthalmology, Nippon Medical School
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- Takao Kazuhiro
- Department of Ophthalmology, Nippon Medical School
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- Igarashi Toru
- Department of Pediatrics, Nippon Medical School
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- Okuda Takahisa
- Division of Legal Medicine, Department of Social Medicine, Nihon University School of Medicine
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- Okada Takashi
- Division of Molecular and Medical Genetics, The Institute of Medical Science, The University of Tokyo
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- Takahashi Hiroshi
- Department of Ophthalmology, Nippon Medical School
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Abstract
<p>Background: Brain-derived neurotrophic factor (BDNF) may be involved in the pathogenesis of glaucoma. BDNF concentrations reported in previous studies have varied widely, and the concentration of BDNF in aqueous humor is unknown. In this study, BDNF concentrations in the aqueous humor of glaucoma patients and control patients were measured with ELISA kits. Methods: This prospective, observational study examined BDNF levels in aqueous humor in 62 eyes of 43 patients who underwent cataract surgery or trabeculectomy (11 glaucoma patients and 32 non-glaucoma cataract patients as controls). BDNF concentrations were examined by 4 different enzyme-linked immunosorbent assay (ELISA) techniques. Results: The mean ± SD patient age was 72.0 ± 10.1 (range 35 to 87) years. Two of the techniques detected no BDNF in aqueous humor in any samples (n=3 and n=9, respectively); the average value was less than zero. An ultrasensitive ELISA kit did not yield reliable measurements. Finally, in an even more sensitive ELISA (Simoa-HD1), performed by an outside contractor, 25 (54.3%) eyes were below the detection limit, including 20 (55.6%) control and 5 (50%) glaucoma cases. For eyes with detectable BDNF, the overall BDNF concentration was 0.158 pg/mL (n=21): 0.196 pg/mL (n=16) in controls and 0.034 pg/mL (n=5) in glaucoma cases. Conclusions: BDNF level in aqueous humor varies widely.</p>
Journal
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- Journal of Nippon Medical School
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Journal of Nippon Medical School 88 (2), 128-132, 2021-04-24
The Medical Association of Nippon Medical School
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Details 詳細情報について
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- CRID
- 1390569468289273728
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- NII Article ID
- 130008037216
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- NII Book ID
- AA11563215
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- ISSN
- 13473409
- 13454676
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- PubMed
- 33980757
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed