Bitterness-masking peptides for epigallocatechin gallate identified through peptide array analysis

DOI Web Site 被引用文献2件 参考文献35件
  • Ito Keisuke
    School of Food and Nutritional Sciences, University of Shizuoka Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Koike Mayu
    School of Food and Nutritional Sciences, University of Shizuoka
  • Kuroda Yuki
    Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Yamazaki-Ito Toyomi
    Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Terada Yuko
    School of Food and Nutritional Sciences, University of Shizuoka Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Ishii Takeshi
    Department of Nutrition, Kobe Gakuin University
  • Nakamura Yoriyuki
    School of Food and Nutritional Sciences, University of Shizuoka
  • Watanabe Tatsuo
    School of Food and Nutritional Sciences, University of Shizuoka
  • Kawarasaki Yasuaki
    School of Food and Nutritional Sciences, University of Shizuoka Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka

抄録

<p>Bitterness-masking agents support the intake of functional food ingredients, which taste bitter. However, evidence-based methodology to find bitterness-masking agents remains to be established. In the present study, focusing on the fact that a bitter tastant-binding compound is expected to have a bitterness-masking effect, several epigallocatechin gallate (EGCg)-binding peptides were identified from the amino acid sequence of ribulose 1,5-bisphosphate carboxylase/oxygenase using peptide array technology. Deletion analysis and alanine scanning analysis of these peptides revealed that electrostatic interaction contribute strongly to the binding mechanism. EGCg-binding peptides identified in this study, namely, MHFRVLAKALR and FTGLKSTSAFPVTRK, successfully suppressed the activation of the bitter-taste receptor hTAS2R39 by administration of EGCg. These results suggest that peptide array technology can be used to screen bitterness-masking peptides.</p>

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (35)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ