Development of microsatellite markers for <i>Saccharina japonica</i> by dual-suppression PCR
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- MAEDA Takashi
- Graduate School of Environmental Science, Hokkaido University
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- YOTSUKURA Norishige
- Field Science Center for Northern Biosphere, Hokkaido University
抄録
Microsatellite loci in Saccharina japonica (Areschoug) C. E. Lane, C. Mayes, Druehl & G. W. Saunders were searched by the dual-suppression PCR method with several modifications. Within 28 SSRs for which both adjacent regions were able to read, the sequence including the complete primer array was confirmed in only three regions. Therefore, it is suggested that the determination of sequences adjacent to both ends of the SSR region become a key for marker development in this method. Accordingly, in this study, from 2 to 9 alleles were found between individuals in 7 loci and they could be detected by electrophoresis that used acrylamide gel.
収録刊行物
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- Algal Resources
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Algal Resources 6 (2), 67-71, 2013
日本応用藻類学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390569711989143424
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- NII論文ID
- 130008048016
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- ISSN
- 24238473
- 18833284
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可