Development of microsatellite markers for <i>Saccharina japonica</i> by dual-suppression PCR

• MAEDA Takashi

  Graduate School of Environmental Science, Hokkaido University

• YOTSUKURA Norishige

  Field Science Center for Northern Biosphere, Hokkaido University

Abstract

Microsatellite loci in Saccharina japonica (Areschoug) C. E. Lane, C. Mayes, Druehl & G. W. Saunders were searched by the dual-suppression PCR method with several modifications. Within 28 SSRs for which both adjacent regions were able to read, the sequence including the complete primer array was confirmed in only three regions. Therefore, it is suggested that the determination of sequences adjacent to both ends of the SSR region become a key for marker development in this method. Accordingly, in this study, from 2 to 9 alleles were found between individuals in 7 loci and they could be detected by electrophoresis that used acrylamide gel.

Journal

• Algal Resources

  Algal Resources 6 (2), 67-71, 2013

  The Japanese Society of Applied Phycology

Keywords

• dual-suppression PCR
• genetic diversity
• microsatellite markers
• polymorphism
• <i>Saccharina japonica</i>

Details

• CRID: 1390569711989143424
• NII Article ID: 130008048016
• DOI: 10.20804/jsap.6.2_67
• ISSN: 24238473
• ISSN: 18833284
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed