Sensing of Acetylcholine-Related Compounds Using Cation-Selective Electrodes

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  • 陽イオン選択性電極を用いたアセチルコリン関連化合物のセンシング
  • ヨウイオン センタクセイ デンキョク オ モチイタ アセチルコリン カンレン カゴウブツ ノ センシング

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Abstract

<p>Acetylcholine (ACh), a cholinergic neurotransmitter, has been widely analyzed by HPLC-electrochemical detection and other methods. However, these method are not durable because it uses a column immobilized with enzymes, such as ACh esterase and choline oxidase, and it does not directly measure ACh because they measure hydrogen peroxide generated by the enzymatic reaction. In this study, we developed an ACh sensor for direct measurement of ACh using an Mg2+ ion selective membrane. The sensor showed good quantitative performance in the range of ACh concentration from 1.00 × 10−4 M - 1.67 × 10−1 M, enabling simple and rapid determination of ACh. By miniaturizing the sensor, quantification was possible from 1.00 × 10−5 M, which improved the sensitivity and reduced the sample volume. The developed sensor can be incorporated into flow injection analysis and HPLC detectors, and is expected to be used in various fields such as screening of drug candidates, rapid identification of toxicants and muscle relaxants, and measurement of related metabolic enzyme activities.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 70 (4.5), 261-265, 2021-04-05

    The Japan Society for Analytical Chemistry

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