Bioelectrical Methane Production with an Ammonium Oxidative Reaction under the No Organic Substance Condition

• Dinh Ha T.T

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University Faculty of Environment, Ho Chi Minh City University of Natural Resources and Environment

• Kambara Hiromi

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Harada Yoshiki

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Matsushita Shuji

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University Agricultural Technology Research Center, Hiroshima Prefectural Technology Research Institute

• Aoi Yoshiteru

  Program of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University

• Kindaichi Tomonori

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Ozaki Noriatsu

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

• Ohashi Akiyoshi

  Department of Civil and Environmental Engineering, Graduate School of Advanced Science and Engineering, Hiroshima University

Bibliographic Information

Other Title

• Bioelectrical methane production with ammonium oxidative reaction under no organic substance condition

Abstract

The present study investigated bioelectrical methane production from CO₂ without organic substances. Even though microbial methane production has been reported at relatively high electric voltages, the amount of voltage required and the organisms contributing to the process currently remain unknown. Methane production using a biocathode was investigated in a microbial electrolysis cell coupled with an NH₄⁺ oxidative reaction at an anode coated with platinum powder under a wide range of applied voltages and anaerobic conditions. A microbial community analysis revealed that methane production simultaneously occurred with biological denitrification at the biocathode. During denitrification, NO₃^– was produced by chemical NH₄⁺ oxidation at the anode and was provided to the biocathode chamber. H₂ was produced at the biocathode by the hydrogen-producing bacteria Petrimonas through the acceptance of electrons and protons. The H₂ produced was biologically consumed by hydrogenotrophic methanogens of Methanobacterium and Methanobrevibacter with CO₂ uptake and by hydrogenotrophic denitrifiers of Azonexus. This microbial community suggests that methane is indirectly produced without the use of electrons by methanogens. Furthermore, bioelectrical methane production occurred under experimental conditions even at a very low voltage of 0.05‍ ‍V coupled with NH₄⁺ oxidation, which was thermodynamically feasible.

Journal

• Microbes and Environments

  Microbes and Environments 36 (2), n/a-, 2021

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• ammonia oxidation
• bio-electricity
• denitrification
• methane production
• microbial community

Details

• CRID: 1390006973362966272
• NII Article ID: 130008053387
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me21007
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 031542275
• PubMed: 34135211
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031542275
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/36/2/36_ME21007/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed