Identification of two tobacco genes encoding MYB3R proteins with repressor function and showing cell cycle-regulated transcript accumulation

• Takatsuka Hirotomo

  School of Biological Science and Technology, College of Science and Engineering, Kanazawa University

• Nomoto Yuji

  School of Biological Science and Technology, College of Science and Engineering, Kanazawa University

• Araki Satoshi

  Division of Biological Science, Graduate School of Science, Nagoya University

• Machida Yasunori

  Division of Biological Science, Graduate School of Science, Nagoya University

• Ito Masaki

  School of Biological Science and Technology, College of Science and Engineering, Kanazawa University

抄録

MYB3R family transcription factors play a central role in the regulation of G2/M-specific gene transcription in Arabidopsis thaliana. Among the members of this family, MYB3R3 and MYB3R5 are structurally closely related and are involved in the transcriptional repression of target genes in both proliferating and quiescent cells. This type of MYB3R repressor is widespread in plants; however, apart from the studies on MYB3Rs in Arabidopsis thaliana, little information about them is available. Here we isolated tobacco cDNA clones encoding two closely related MYB3R proteins designated as NtmybC1 and NtmybC2 and determined the nucleotide sequences of the entire coding regions. Phylogenetic analysis suggested that NtmybC1 and NtmybC2 can be grouped into a conserved subfamily of plant MYB3Rs that also contains MYB3R3 and MYB3R5. When transiently expressed in protoplasts prepared from tobacco BY-2 cells, NtmybC1 and NtmybC2 repressed the activity of target promoters and blocked promoter activation mediated by NtmybA2, a MYB3R activator from tobacco. Unlike MYB3R3 and MYB3R5, NtmybC1 and NtmybC2 showed cell cycle-regulated transcript accumulation. In synchronized cultures of BY-2 cells, mRNAs for both NtmybC1 and NtmybC2 were preferentially expressed during the G2 and M phases, coinciding with the expression of NtmybA2 and G2/M-specific target genes. These results not only broadly confirm our fundamental view that this type of MYB3R protein acts as transcriptional repressor of G2/M-specific genes but also suggest a possible divergence of MYB3R repressors in terms of the mechanisms of their action and regulation.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 38 (2), 269-275, 2021-06-25

  日本植物バイオテクノロジー学会

キーワード

• BY-2 cells
• Cell cycle
• MYB3R
• synchronized culture
• transcriptional regulation

詳細情報

• CRID: 1390569955047810560
• NII論文ID: 130008056767
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.21.0224a
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 031558186
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031558186
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/38/2/38_21.0224a/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可