Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In <i>C. elegans</i> Strains
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- Nishida Kei
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Tsuchiya Kenta
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Obinata Hiroyuki
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Onodera Shizuka
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Honda Yu
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Lai Yen-Cheng
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Haruta Nami
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
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- Sugimoto Asako
- Laboratory of Developmental Dynamics, Graduate School of Life Sciences, Tohoku University
Bibliographic Information
- Other Title
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- Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains
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Abstract
<p>Most organisms have multiple α- and β-tubulin isotypes that likely contribute to the diversity of microtubule (MT) functions. To understand the functional differences of tubulin isotypes in Caenorhabditis elegans, which has nine α-tubulin isotypes and six β-tubulin isotypes, we systematically constructed null mutants and GFP-fusion strains for all tubulin isotypes with the CRISPR/Cas9 system and analyzed their expression patterns and levels in adult hermaphrodites. Four isotypes—α-tubulins TBA-1 and TBA-2 and β-tubulins TBB-1 and TBB-2—were expressed in virtually all tissues, with a distinct tissue-specific spectrum. Other isotypes were expressed in specific tissues or cell types at significantly lower levels than the broadly expressed isotypes. Four isotypes (TBA-5, TBA-6, TBA-9, and TBB-4) were expressed in different subsets of ciliated sensory neurons, and TBB-4 was inefficiently incorporated into mitotic spindle MTs. Taken together, we propose that MTs in C. elegans are mainly composed of four broadly expressed tubulin isotypes and that incorporation of a small amount of tissue-specific isotypes may contribute to tissue-specific MT properties. These newly constructed strains will be useful for further elucidating the distinct roles of tubulin isotypes.</p><p>Key words: tubulin isotypes, microtubules, C. elegans</p>
Journal
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- Cell Structure and Function
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Cell Structure and Function 46 (1), 51-64, 2021
Japan Society for Cell Biology
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Details 詳細情報について
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- CRID
- 1390288535620954368
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- NII Article ID
- 130008059056
- 130008035994
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- NII Book ID
- AA0060007X
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- ISSN
- 13473700
- 03867196
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- NDL BIB ID
- 032032605
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- PubMed
- 33967119
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed