Deficiency of Type I Platelet-Activating Factor-Acetylhydrolase Catalytic Subunits Causes an Increase in Body Weight
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- Nakamura Yasuhiro
- Faculty of Pharma-sciences, Teikyo University
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- Yoshida Momoko
- Pharma Cluster Co., Ltd.
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- Tanigawa Kazunari
- Faculty of Pharma-sciences, Teikyo University
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- Harada Ayako
- Faculty of Pharma-sciences, Teikyo University
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- Kihara-Negishi Fumiko
- Faculty of Pharma-sciences, Teikyo University
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- Maruyama Keiji
- Faculty of Pharma-sciences, Teikyo University
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- Karasawa Ken
- Faculty of Pharma-sciences, Teikyo University
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Abstract
<p>Type I platelet-activating factor-acetylhydrolase (PAF-AH) forms a complex consisting of two catalytic subunits (α1 and/or α2) with a regulatory subunit (β). Although this protein was discovered as an enzyme that degrades an acetyl ester linked at the sn-2 position of platelet-activating factor (PAF), its physiological function remains unknown. In this study, to examine whether knockout mice lacking the catalytic subunits of this enzyme showed a different phenotype from that of wild-type mice, we measured and compared the body weights of knockout mice and control mice. The body weights of knockout mice were significantly increased compared to those of the control mice during 6 to 20 weeks from birth. Food intake was also significantly increased in knockout mice compared with control mice during these periods. Since a decrease in testis weight was reported in the knockout mice, we expected a decrease in testosterone levels. We measured and compared the amounts of testosterone in the serum and testis of knockout and control mice using liquid chromatography-tandem mass spectrometry, and found that testosterone levels in both the serum and testis were significantly decreased in the knockout mice compared with the control mice. These results suggest that a deficiency of type I PAF-AH catalytic subunits causes an increase in body weight, in part, due to reduced testosterone levels in male mice.</p>
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 44 (7), 920-925, 2021-07-01
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390570022236838272
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- NII Article ID
- 130008060354
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 031534912
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- PubMed
- 34193688
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed