Elevation of the serotonin-derived quinone, tryptamine-4,5-dione, in the intestine of ICR mice with dextran sulfate-induced colitis

DOI Web Site 33 References Open Access
  • Suga Naoko
    Graduate School of Human Science and Environment, University of Hyogo
  • Murakami Akira
    Graduate School of Human Science and Environment, University of Hyogo Research Institute for Food and Nutritional Sciences, University of Hyogo
  • Arimitsu Hideyuki
    Graduate School of Human Science and Environment, University of Hyogo Research Institute for Food and Nutritional Sciences, University of Hyogo
  • Shiogama Kazuya
    Department of Diagnostic Pathology II, Fujita Health University School of Medicine
  • Tanaka Sarasa
    Graduate School of Human Science and Environment, University of Hyogo Research Institute for Food and Nutritional Sciences, University of Hyogo
  • Ito Mikiko
    Graduate School of Human Science and Environment, University of Hyogo Research Institute for Food and Nutritional Sciences, University of Hyogo
  • Kato Yoji
    Graduate School of Human Science and Environment, University of Hyogo Research Institute for Food and Nutritional Sciences, University of Hyogo

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Abstract

<p>Inflammatory bowel diseases, including Crohn’s disease and ulcerative colitis, are chronic inflammatory disorders associated with oxidative stress. The intestines produce 5-hydroxytryptamine that may negatively affect disease state under inflammatory conditions when overproduced. 5-Hydroxytryptamine is a sub­strate for myeloperoxidase and is converted into reactive tryptamine-4,5-dione. Here, an experimental colitis model was established through oral administration of 5% dextran sulfate sodium to ICR mice for 7 days. Furthermore, the formation of tryptamine-4,5-dione in the colorectal mucosa/submucosa and colorectal tissue was analyzed by chemical and immunochemical methodologies. First, free tryptamine-4,5-dione in the homogenate was chemically trapped by o-phenylenediamine and analyzed as the stable phenazine derivative. Tryptamine-4,5-dione locali­zation as adducted proteins in the colorectal tissue was immunohistochemically confirmed, and as demonstrated by both methods, this resulted in the significant increase of tryptamine-4,5-dione in dextran sulfate sodium-challenged mice compared with control mice. Immunohistochemical staining confirmed tryptamine-4,5-dione-positive staining at the myeloperoxidase accumulation site in dextran sulfate sodium-challenged mice colorectal tissue. The tryptamine-4,5-dione locus in the mice was partly matched with that of a specific marker for myeloperoxidase, halogenated tyrosine. Overall, the results possibly indicate that tryptamine-4,5-dione is generated by neutrophil myeloperoxidase in inflammatory tissue and may contribute to the development of inflammatory bowel disease.</p>

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