Selective Stimulation of a Target Neuron in Micropatterned Neuronal Circuits Using a Pair of Needle Electrodes

• HATTORI Kouhei

  Faculty of Science and Engineering, Waseda University

• KURAKAKE Hekiru

  Faculty of Science and Engineering, Waseda University

• IMAI Junko

  Faculty of Science and Engineering, Waseda University

• HASHIMOTO Takuya

  Faculty of Science and Engineering, Waseda University

• ISHIDA Mihoko

  Faculty of Science and Engineering, Waseda University

• SATO Koki

  Faculty of Science and Engineering, Waseda University

• TAKAHASHI Honoka

  Faculty of Science and Engineering, Waseda University

• OGUMA Soichiro

  Faculty of Science and Engineering, Waseda University

• YAMAMOTO Hideaki

  Research Institute of Electrical Communication, Tohoku University

• HIRANO-IWATA Ayumi

  Research Institute of Electrical Communication, Tohoku University WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University

• TANII Takashi

  Faculty of Science and Engineering, Waseda University

Abstract

Neurostimulation is an essential technique to trigger and modulate the spatiotemporal activity of local neuronal circuits. Current stimulation methods have a trade-off relationship among aiming precision, temporal resolution, and noninvasiveness, making it difficult to stimulate and monitor a single target neuron for a long term. Here, we show that a method using two needle electrodes in combination of micropatterning techniques provides new possibilities for targeting and stimulating a single neuron selectively. Results of physiological experiments as well as analog circuit simulation reveal that two needle electrodes can stimulate a target neuron selectively by placing the two needle electrodes in proximity to and to straddle the target neuron, and that the steepness of voltage applied to two needle electrodes is important for the target neuron to fire at a low voltage. The proposed method enables a noninvasive stimulation suitable for measuring long-term activity of local neuronal circuits.

Journal

• Electrochemistry

  Electrochemistry 89 (4), 348-354, 2021-07-05

  The Electrochemical Society of Japan

Keywords

• Neuron
• Two Needle Electrodes
• Selective Stimulation
• LTspice

Details

• CRID: 1390570078121632384
• NII Article ID: 130008062336
• DOI: 10.5796/electrochemistry.21-00032
• ISSN: 21862451
• ISSN: 13443542
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/89/4/89_21-00032/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed