Development of a method for quantification of serum dehydroepiandrosterone sulfate by stable isotope-dilution MALDI-MS
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- Ogawa Shoujiro
- Faculty of Pharmaceutical Sciences, Tokyo University of Science Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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- Nakamura Masahiro
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Satoh Mamoru
- Division of Clinical Mass Spectrometry, Chiba University Hospital
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- Ishige Takayuki
- Division of Laboratory Medicine, Chiba University Hospital
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- Nomura Fumio
- Division of Clinical Genetics, Chiba University Hospital
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- Sugiura Yuki
- Department of Biochemistry, Keio University School of Medicine
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- Higashi Tatsuya
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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<p>The quantification of dehydroepiandrosterone sulfate (DHEAS) in the human circulation might be of diagnostic help for several diseases, such as diabetes, depression, Alzheimer’s disease, osteoporosis and chronic heart failure. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) could be an effective approach to quantify the serum DHEAS due to its high specificity, handleability, rapid analysis and ruggedness. Nonetheless, to the best of our knowledge, no MALDI-MS assay has been reported for the quantification of DHEAS in biological samples. In this study, we developed and validated a method to quantify DHEAS in human serum by MALDI-MS using a stable isotope-dilution technique with 2H4-DHEAS as an internal standard. This method enabled the reproducible quantification of the serum DHEAS (intra- and inter-assay relative standard deviations, 9.3% or lower) with a small sample volume (20 μL) and effort-saving pretreatment (only deproteinization and crystallization with 9-aminoacridine). The measurable range was 0.250–5.00 μg/mL and the analysis time after the sample preparation was 3 min. The serum DHEAS concentrations measured by the newly-developed MALDI-MS method agreed well with those by liquid chromatography/electrospray ionization-MS/MS; this result demonstrated that the developed MALDI-MS method yielded reliable results.</p>
収録刊行物
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- Medical Mass Spectrometry
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Medical Mass Spectrometry 5 (1), 22-27, 2021-06-25
一般社団法人 日本医用マススペクトル学会
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詳細情報 詳細情報について
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- CRID
- 1390851553098462080
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- NII論文ID
- 130008062732
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- NII書誌ID
- AA12788254
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- ISSN
- 2432745X
- 24327441
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- NDL書誌ID
- 031588665
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可