Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

• ABDALKADER Rodi

  Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University

• CHALECKIS Romanas

  Gunma University Initiative for Advanced Research (GIAR), Gunma University Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute

• MEISTER Isabel

  Gunma University Initiative for Advanced Research (GIAR), Gunma University Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute

• ZHANG Pei

  Gunma University Initiative for Advanced Research (GIAR), Gunma University Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute

• WHEELOCK Craig E.

  Gunma University Initiative for Advanced Research (GIAR), Gunma University Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute

• KAMEI Ken-ichiro

  Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University Wuya College of Innovation, Shenyang Pharmaceutical University

Abstract

Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

Journal

• Analytical Sciences

  Analytical Sciences 37 (7), 1049-1052, 2021-07-10

  The Japan Society for Analytical Chemistry

Keywords

• Hepatocyte
• extracellular metabolites
• LC-MS

Details

• CRID: 1390007216692897280
• NII Article ID: 130008064007
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.20n032
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 031566821
• PubMed: 33342928
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031566821
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.20N032.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.20N032/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/37/7/37_20N032/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022158884
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed