Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice

• Kashiwakura Jun-ichi

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Yoshihara Mari

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Saitoh Kodai

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Kagohashi Kota

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Sasaki Yuto

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Kobayashi Fuki

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Inagaki Iori

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Kitai Yuichi

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Muromoto Ryuta

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

• Matsuda Tadashi

  Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University

Abstract

Background: Propolis is a resinous mixture produced by honey bees that contains cinnamic acid derivatives and flavonoids. Although propolis has been reported to inhibit mast cell functions and mast cell-dependent allergic responses, the effect of propolis on basophil biology remains unknown. This study aimed to investigate the inhibitory effect of propolis on FcεRI-mediated basophil activation. Methods: To determine the inhibitory effect of propolis on basophil activation in vitro, cytokine production and FcεRI signal transduction were analyzed by ELISA and western blotting, respectively. To investigate the inhibitory effect of propolis in vivo, IgE-CAI and a food allergy mouse model were employed. Results: Propolis treatment resulted in the suppression of IgE/antigen-induced production of IL-4, IL-6 and IL-13 in basophils. Phosphorylation of FcεRI signaling molecules Lyn, Akt and ERK was inhibited in basophils treated with propolis. While propolis did not affect the basophil population in the treated mice, propolis did inhibit IgE-CAI. Finally, ovalbumin-induced intestinal anaphylaxis, which involves basophils and basophil-derived IL-4, was attenuated in mice prophylactically treated with propolis. Conclusions: Taken together, these results demonstrate the ability of propolis to suppress IgE-dependent basophil activation and basophil-dependent allergic inflammation. Therefore, prophylactic treatment with propolis may be useful for protection against food allergic reactions in sensitive individuals.

Journal

• Allergology International

  Allergology International 70 (3), 360-367, 2021

  Japanese Society of Allergology

Keywords

• Basophils
• Food allergy
• IgE
• IL-4
• Propolis

Details

• CRID: 1390570166645968128
• NII Article ID: 130008064059
• DOI: 10.1016/j.alit.2020.11.005
• ISSN: 14401592
• ISSN: 13238930
• HANDLE: 2115/82067
• Web Site: https://api.elsevier.com/content/article/PII:S1323893020301490?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S1323893020301490?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2022173663
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed