Effects of Calcium Lactate on the Development of Chicken Embryos in a Shell-less Culture System up to Day Seventeen of Incubation

  • Obara Katsuya
    Takanedai Animal Clinic Graduate School of Science and Technology, University of Tsukuba
  • Obara (Henmi) Chizuka
    Faculty of Veterinary Medicine, Imabari Campus, Okayama University of Science
  • Naito Mitsuru
    National Institute of Agrobiological Sciences
  • Mitsui Ikki
    Faculty of Veterinary Medicine, Imabari Campus, Okayama University of Science
  • Une Yumi
    Faculty of Veterinary Medicine, Imabari Campus, Okayama University of Science
  • Asano Atsushi
    Faculty of Life and Environmental Sciences, University of Tsukuba
  • Tajima Atsushi
    Faculty of Life and Environmental Sciences, University of Tsukuba

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<p>This study examined the effects of calcium lactate on the development of chicken embryos in a shell-less culture system (cSLCS) up to the seventeenth day of incubation. In the presence of calcium lactate, a significant reduction in embryo viability was observed during the first week of incubation in cSLCS. On day 17 of embryo development, no significant difference was observed in the blood plasma calcium concentration or tibia bone density between cSLCS and intact control embryos, whereas the tibia length was significantly shorter in cSLCS embryos than in the intact control. These results suggest that calcium lactate supplementation in cSLCS supports bone formation in developing chicken embryos, but has adverse effects on the viability of embryos, particularly during the first week of embryo development.</p>

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