Selectivity Determinant of Ancestor-like Prokaryotic Calcium Channel

• SHIMOMURA Takushi

  Division of Biophysics and Neurobiology, National Institute for Physiological Sciences

• IRIE Katsumasa

  Cellular and Structural Physiology Institute (CeSPI), Nagoya University Present Address: School of Pharmacy, Wakayama Medical University

Bibliographic Information

Other Title

• 細菌の祖先型イオンチャネルから探る，普遍的なカルシウム選択機構
• サイキン ノ ソセンガタ イオンチャネル カラ サグル,フヘンテキ ナ カルシウム センタク キコウ

Abstract

Voltage-dependent Ca²⁺ channels (Cavs) are indispensable for coupling action potentials with Ca²⁺ signaling. We report the first identification of a native prokaryotic Cav, CavMr, whose selectivity filter contains a smaller number of negatively charged residues than that of artificial prokaryotic Cavs. A relative mutant whose selectivity filter was replaced with that of CavMr exhibits high Ca²⁺ selectivity. The glycine residue of the CavMr selectivity filter is a determinant for Ca²⁺ selectivity. This glycine residue is well conserved among subdomains I and III of eukaryotic Cavs, which provide new insight into Ca²⁺ selectivity mechanism conserved from prokaryotes to eukaryotes.

Journal

• Seibutsu Butsuri

  Seibutsu Butsuri 61 (4), 223-226, 2021

  The Biophysical Society of Japan General Incorporated Association

Keywords

• ion channel
• ion selectivity
• electrophysiology
• structural biology

Details

• CRID: 1390007392211401472
• NII Article ID: 130008068996
• NII Book ID: AN00129693
• DOI: 10.2142/biophys.61.223
• ISSN: 13474219
• ISSN: 05824052
• NDL BIB ID: 031678642
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031678642
• Web Site: https://www.jstage.jst.go.jp/article/biophys/61/4/61_223/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed