Construction of transcription factor gene deletion library of <i>Aspergillus luchuensis</i>
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- Yamada Osamu
- National Research Institute of Brewing
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- Nishibori Naoko
- National Research Institute of Brewing Hiroshima University Genome Editing Innovation Center
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- Hayashi Risa
- National Research Institute of Brewing
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- Arima Toshihide
- Department of Life and Environmental Sciences, Prefectural University of Hiroshima
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- Mizutani Osamu
- National Research Institute of Brewing Department of Bioscience and Biotechnology, University of the Ryukyus
抄録
<p>We selected 96 genes of Aspergillus luchuensis for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using Agrobacterium-mediated transformation (AMT) of A. luchuensis RIB 2604 ΔligD strains. We obtained only heterokaryonic strains after deletions of adaB, anBH1, hacA, hapB, hsf1, metR, and sonC gene, and additionally, could not obtain deletion strains for genes abaA and mcmA. The deletion strains will be available through our website (https://www.nrib.go.jp).</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 67 (3), 118-123, 2021
公益財団法人 応用微生物学・分子細胞生物学研究奨励会