Control of Stem Cell Fate and Function by Engineered Surface Topography Using Metal Additive Manufacturing Technology

• MATSUGAKI Aira

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• NAKAMURA Fumihito

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• TAKEHANA Ryo

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• TODO Tsubasa

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• FUKUSHIMA Ryo

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• MATSUZAKA Tadaaki

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• OZASA Ryosuke

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• ISHIMOTO Takuya

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• MIYABE Sayaka

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

• NAKANO Takayoshi

  Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

Bibliographic Information

Other Title

• 金属 3D プリンティングによる表面構造制御とそれに基づく幹細胞制御
• キンゾク 3Dプリンティング ニ ヨル ヒョウメン コウゾウ セイギョ ト ソレニ モトズク カンサイボウ セイギョ

Abstract

The structural regeneration of biological tissue is imperative for recovery of the organ function during tissue healing process. The mechanical and biological function of bone tissue is governed by the oriented microstructure of collagen/apatite matrix. Control of unidirectional cell alignment triggers the subsequent oriented bone matrix organization. However, the development of biomedical devices equipped with ideal design for microstructural recovery of bone tissue is not yet fully achieved. Mesenchymal stem cells （MSCs） play important roles in bone tissue regeneration, which have been considered as a promising therapeutic target easily isolatable form the patients. Here, we propose additive manufacturing（AM）technology as a powerful tool for control of the differentiation fate and the function of mesenchymal stem cells. MSCs aligned along the grooved structure fabricated by selective laser melting. Moreover, the aligned MSCs showed upregulated expression of BGLAP, an important osteogenic differentiation marker gene. The results indicate the technological advances of AM process, which realize the functional bone tissue regeneration from MSCs.

Journal

• Journal of Smart Processing

  Journal of Smart Processing 10 (4), 261-264, 2021-07-10

  Smart Processing Society for Materials, Environment & Energy (High Temperature Society of Japan)

Keywords

• Additive Manufacturing
• Mesenchymal Stem Cell
• Bone Tissue Microstructure
• Implant
• Surface Topography

Details

• CRID: 1390007437192522368
• NII Article ID: 130008069937
• NII Book ID: AA12553487
• DOI: 10.7791/jspmee.10.261
• ISSN: 21871337
• ISSN: 2186702X
• NDL BIB ID: 031614254
• Web Site: http://id.ndl.go.jp/bib/031614254
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031614254
• Web Site: https://www.jstage.jst.go.jp/article/jspmee/10/4/10_261/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed