Simultaneous Determination of Five Bile Acids as Potential Biomarkers for Alzheimer’s Disease in Mouse Brain and Plasma

• KOIKE Shin

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• MIYAJI Yusuke

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• SANO Hinako

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• AIKAWA Natsuki

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• KAI Masayuki

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• KASAHARA Sakura

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• SUZUKI Toshihiro

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• NISHIMOTO-KUSUNOSE Shoichi

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

• OGASAWARA Yuki

  Department of Analytical Biochemistry, Meiji Pharmaceutical University

Abstract

In this study, we developed an analytical method using LC-MS/MS for the simultaneous determination of five bile acids (BAs) that have been recently reported as candidate diagnostic biomarkers for Alzheimer’s disease (AD) or AD related factors in the brain. The measurement of BAs in the brains of healthy mice led to the determination of candidate diagnostic markers for AD, such as cholic acid and deoxycholic acid, and other bile acids, such as chenodeoxycholic acid noted for the ameliorating effect on the symptoms of AD. Significant positive correlations were observed between the brain and plasma concentrations of four BAs in healthy young mice. These results indicate that the BA level in the brain may be estimated by the corresponding BA level in the plasma. Thus, our study suggested that the proposed method for the analysis of the five bile acids would aid in the diagnosis of AD or in studies that use AD model mice.

Journal

• Analytical Sciences

  Analytical Sciences 37 (8), 1165-1170, 2021-08-10

  The Japan Society for Analytical Chemistry

Keywords

• Alzheimer’s disease
• bile acid
• biomarker
• LC-MS/MS
• mouse brain
• simultaneous determination

Details

• CRID: 1390851940045736960
• NII Article ID: 130008072575
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.20p429
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 031618116
• PubMed: 33518588
• Web Site: http://id.ndl.go.jp/bib/031618116
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031618116
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.20P429.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.20P429/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/37/8/37_20P429/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022185105
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed