Developmental toxicity of cefixime on the embryonic ear of zebrafish

• Bao CHAO

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

• Nini BU

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

• Shuaiyu WANG

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

• Xiaoyu YIN

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

• Hao CHEN

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

• Wu DONG

  Collage of Animal Science and Technology, Inner Mongolia University for Nationalities

抄録

Cefixime is the third generation cephalosporins, which is famous on high efficiency and low toxicity with widely used in clinic. However, the embryonic toxicity of cefixime needs to be further studied. In this study, zebrafish embryos were used as an animal model to evaluate the embryotoxicity of cefixime. Zebrafish embryos were exposed to cefixime (0-100 μ g/mL) from 4 hours after fertilization (4hpf), until observation. Cefixime had no significant effects on the mortality of zebrafish embryos, but the hatching rate was decreased significantly (P < 0.05) at 96 hpf. The mortality rate was significant raised (P < 0.05), swimming distance were deceased and tilted to one side (P < 0.05) at 120 hpf after 100 μ g / mL cefixime exposure. Also, the fluorescent spots of hair cells on the side of zebrafish larvae were decreased significantly with the increase of concentration by DASPEI fluorescence staining. Compare with control group, the otolith area were significantly decreased about 50% after Cefixime exposure (P < 0.01 or 0.05). Cefixime also down-regulated eya1 mRNA expression and increased stat3 mRNA expressions. Our results show that cefixime has a toxic effect on zebrafish development, especially on ear development. It is recommended to use with caution for pregnant women and children. Keywords：Cefixime; Ototoxicity; Otoliths; Hair cells; Zebrafish embryos

収録刊行物

• 日本毒性学会学術年会

  日本毒性学会学術年会 48.1 (0), P-108S-, 2021

  日本毒性学会

詳細情報

• CRID: 1390570486221677312
• NII論文ID: 130008073582
• DOI: 10.14869/toxpt.48.1.0_p-108s
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可