Interkinetic nuclear migration in the mouse embryonic ureteric epithelium: Possible implication for congenital anomalies of the kidney and urinary tract

• Tomoyuki Motoya

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Noriko Ogawa

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Tetsuya Nitta

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Ashiq Mahmood Rafiq

  Center for the Promotion of Project Research, Organization for Research Shimane University Matsue Shimane Japan

• Esrat Jahan

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Motohide Furuya

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Akihiro Matsumoto

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

• Jun Udagawa

  Division of Anatomy and Cell Biology, Department of Anatomy Shiga University of Medical Science Otsu Shiga Japan

• Hiroki Otani

  Department of Developmental Biology, Faculty of Medicine Shimane University Izumo Shimane Japan

抄録

Abstract Interkinetic nuclear migration (INM) is a phenomenon in which progenitor cell nuclei migrate along the apico-basal axis of the pseudostratified epithelium, which is characterized by the presence of apical primary cilia, in synchrony with the cell cycle in a manner of apical mitosis. INM is suggested to regulate not only stem/progenitor cell proliferation/differentiation but also organ size and shape. INM has been reported in epithelia of both ectoderm and endoderm origin. We examined whether INM exists in the mesoderm-derived ureteric epithelium. At embryonic day (E) 11.5, E12.5 and E13.5, C57BL/6J mouse dams were injected with 5-bromo-2’-deoxyuridine (BrdU) and embryos were killed 1, 2, 4, 6, 8, 10 and 12 h later. We immunostained transverse sections of the ureter for BrdU, and measured the position of BrdU (+) nuclei in the ureteric epithelia along the apico-basal axis at each time point. We analyzed the distribution patterns of BrdU (+) nuclei in histograms using the multidimensional scaling. Changes in the nucleus distribution patterns suggested nucleus movement characteristic of INM in the ureteric epithelia, and the mode of INM varied throughout the ureter development. While apical primary cilia are related with INM by providing a centrosome for the apical mitosis, congenital anomalies of the kidney and urinary tract (CAKUT) include syndromes linked to primary ciliary dysfunction affecting epithelial tubular organs such as kidney, ureter, and brain. The present study showed that INM exists in the ureteric epithelium and suggests that INM may be related with the CAKUT etiology via primary ciliary protein function.

収録刊行物

• 日本先天異常学会会報

  日本先天異常学会会報 56 (3), 127-134, 2016

  日本先天異常学会

キーワード

• Developmental Biology
• Embryology
• General Medicine
• Pediatrics, Perinatology and Child Health

詳細情報

• CRID: 1390007750044732800
• NII論文ID: 130008079235
• DOI: 10.24540/cgafa.56.3_127
• DOI: 10.1111/cga.12150
• ISSN: 24331503
• ISSN: 17414520
• ISSN: 00372285
• ISSN: 09143505
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fcga.12150
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/cga.12150
• Web Site: https://search.jamas.or.jp/link/ui/2018321559
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
  • KAKEN
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