Inhibiting effect of synthetic urine on the growth and mobility of <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Proteus mirabilis</i> and <i>Klebsiella pneumoniae</i> shifts to a promoting effect during culture

DOI Open Access
  • Koudounas Sofoklis
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Skincare Science, Graduate School of Medicine, The University of Tokyo
  • Minematsu Takeo
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Skincare Science, Graduate School of Medicine, The University of Tokyo
  • Mugita Yuko
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Tomida Sanai
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Nakai Ayano
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Nakagami Gojiro
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Sanada Hiromi
    Global Nursing Research Center, Graduate School of Medicine, The University of Tokyo Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo

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Other Title
  • <i>Pseudomonas aeruginosa</i>，<i>Escherichia coli</i>，<i>Proteus mirabilis</i> および <i>Klebsiella pneumoniae</i> の増殖および運動性への人工尿の抑制効果は培養中に促進的効果に変化する

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Abstract

Incontinence-associated dermatitis (IAD) is caused from the repeated exposure to urine/faeces and reduces quality of life. However, the underlying mechanisms are poorly understood. This study explored the effects of urine on the growth and motility of common uropathogens. Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Klebsiella pneumoniae were grown for 24 hrs at 37℃ in Luria-Bertani (LB) medium supplemented with various concentrations of synthetic urine. Bacterial growth was monitored over time by measuring absorbance at 600nm. Swimming, swarming, and twitching motilities were determined in cultures of LB with (1: 1 ratio) and without synthetic urine. Results demonstrated that bacterial growth was inhibited during early phase (0-6 hrs), and enhanced after 8 hrs. For P. mirabilis and K. pneumoniae, the increase in growth was in a dose-dependent manner. Swimming motility of P. mirabilis was inhibited. This study suggested the different effect of fresh and infectious urine on the bacterial growth, which is important to understand the mechanisms of IAD development due to urine incontinence.

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