Effects of omega-7 palmitoleic acids on skeletal muscle differentiation in a hyperglycemic condition
-
- TOKUNAGA Yayoi
- Graduate School of Agriculture and Life Sciences, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
-
- YOSHIZAKI Hitomi
- Department of Molecular Nutrition, Faculty of Human Life Sciences, Jissen Women’s University, 4-1-1 Osakaue, Hino, Tokyo 191-8510, Japan
-
- TORIUMI Akiyo
- Graduate School of Medical and Dental Sciences, Comprehensive Reproductive Medicine, National University Corporation Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
-
- KAWAHARADA Ritsuko
- Department of Health and Nutrition, Takasaki University of Health and Welfare, 37-1 Nakaorui-machi, Takasaki, Gunma 370-0033, Japan
-
- ISHIDA Chisato
- Department of Nutrition, Japanese Haramachi Red Cross Hospital, 698 Haramachi, Agatsumagun, Higashiagatsuma-machi, Gunma 377-0801, Japan
-
- HORI Masatoshi
- Graduate School of Agriculture and Life Sciences, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
-
- NAKAMURA Akio
- Department of Molecular Nutrition, Faculty of Human Life Sciences, Jissen Women’s University, 4-1-1 Osakaue, Hino, Tokyo 191-8510, Japan
この論文をさがす
抄録
<p>Maternal obesity and diabetes are known to be involved in fetal myogenesis, but the later stages of myogenesis are not well understood. In this study, we investigated the influence of a hyperglycemic environment on L6 skeletal myoblast differentiation and the function of omega-7 palmitoleic acids. Exposure to a high concentration of glucose (25 mM) in high-glucose culture medium (HG) increased the expression of myogenic genes (MyoD, Myogenin, MRF4, Myhc2x, and Myhc2a) and the synthesis of myosin. HG also activated the PI3K/AKT pathway revealed muscle cell differentiation. Furthermore, the levels of reactive oxygen species (ROS) and an inflammatory cytokine (Tnfaip3; tumor necrosis factor alpha-induced protein 3), which are crucial for the growth and differentiation of skeletal muscle, were increased by HG. Palmitoleic acids suppressed the expression levels of myogenic regulatory genes and increased the expression level of a cell proliferation-related gene (Pax3). Trans-palmitoleic acid and eicosapentaenoic acid (TPA and EPA) increased the phosphorylation level of MAPK/ERK1/2 and downregulated ROS generation and Tnfaip3 expression. In contrast, cis-palmitoleic acid inactivated MAPK/ERK1/2, leading to increased ROS generation. In conclusion, a hyperglycemic environment mediated by HG induced excessive muscle differentiation. Palmitoleic acids inhibited myoblast differentiation by downregulating muscle-specific genes. Moreover, trans-palmitoleic acids may have beneficial antioxidant and/or anti-inflammatory effects in cells.</p>
収録刊行物
-
- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
-
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 83 (9), 1369-1377, 2021
公益社団法人 日本獣医学会