Angiogenic effects of high molecular weight fucoidan in a mouse ischemic limb model

• Morita Maki

  Department of Plastic and Reconstructive Surgery, Tottori University Faculty of Medicine

• Sugihara Shinobu

  Health Service Center, Shimane University

• Inoue Yumiko

  Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science

• Suyama Yoshiko

  Department of Plastic and Reconstructive Surgery, Tottori University Faculty of Medicine

• Notsu Tomomi

  Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science

• Yamamoto Yasutaka

  Yasutaka Yamamoto cardio-vascular clinic

• Sakaguchi Hiromi

  Department of Radiology, Tottori University Faculty of Medicine

• Fukuoka Kohei

  Department of Plastic and Reconstructive Surgery, Tottori University Faculty of Medicine

• Endo Yusuke

  Advanced Medicine, Innovation and Clinical Research Center, Tottori University Hospital

• Koga Atsuro

  Organization for Research Initiative and Promotion, Tottori University

• Kawamoto Hitoshi

  Marine Products Kimuraya Co., Ltd.

• Funakoshi Minoru

  Marine Products Kimuraya Co., Ltd.

• Miki Yasunari

  Marine Products Kimuraya Co., Ltd.

• Yagi Shunjiro

  Department of Plastic and Reconstructive Surgery, Tottori University Faculty of Medicine

• Ninomiya Haruaki

  Department of Biological Regulation, Tottori University Faculty of Medicine

• Hisatome Ichiro

  Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science

Abstract

Background：The biological actions of fucoidan depend on its molecular weight. 20-30 kDa fucoidan has been reported to stimulate angiogenesis in ischemic limbs. We purified very high molecular weight fucoidan (HMWF) from mozuku (brown algae of the Okinawan coontail family) to assess its effect on angiogenesis. Methods and Results：We examined the angiogenic effects of mozuku HMWF (300 kDa) and akamoku (Sargassum seaweed) HMWF (80 kDa) in a mouse ischemic limb model by measuring laser Doppler blood flow (LDBF) and capillary density. We also studied the angiogenic actions of mozuku HMWF administered pre- and post-ischemia as compared to post-ischemia treatment. Mozuku HMWF increased both LDBF and capillary density in the ischemic leg, whereas akamoku HMWF did not. Treatment with mozuku HMWF pre- and post-ischemia increased both LDBF and capillary density, which was not seen in post-ischemia treatment alone. Conclusions：This study demonstrated the therapeutic effect of pre- and post-ischemia treatment with mozuku HMWF in ischemic limbs, and the timing of administration is important for its angiogenic activity.

Journal

• Vascular Failure

  Vascular Failure 4 (2), 61-67, 2021-08-31

  Japan Society for Vascular Failure

Keywords

• HMWF
• LMWF
• Angiogenesis
• PAD

Details

• CRID: 1390570784699120896
• NII Article ID: 130008086112
• DOI: 10.30548/vascfail.4.2_61
• ISSN: 24324477
• Web Site: https://www.jstage.jst.go.jp/article/vascfail/4/2/4_61/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022174571
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed