Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism
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- SAHASHI Yuma
- Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan Present address: Nagoya City Agricultural Center dela Farm, 2872-3 Aza Kuroishi, Oaza Hirabari, Tenpaku, Nagoya, Aichi 468-0021, Japan
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- OSHIMA Masaaki
- Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
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- YAMAGISHI Junjiro
- Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
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- MURAMATSU Chieko
- Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
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- SHIMIZU Kaori
- Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
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- INOSHIMA Yasuo
- Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan Education and Research Center for Food Animal Health, Gifu University (GeFAH), 1-1 Yanagido, Gifu, Gifu 501-1193, Japan The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan Joint Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
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抄録
<p>Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.</p>
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 83 (11), 1730-1734, 2021
公益社団法人 日本獣医学会