Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling

• Nagata Kosuke

  Natural History Sciences, Hokkaido University

• Bajo Ken-ichi

  Natural History Sciences, Hokkaido University

• Mitomo Hideyuki

  Research Institute for Electronic Science (RIES), Hokkaido University Global Station for Soft Matter, Global Institution for Collaborative Research and Education, Hokkaido University

• Fujita Ryosuke

  Laboratory of Sanitary Entomology, Department of Bioresource Science, Faculty of Agriculture, Kyushu University

• Uehara Ryota

  Graduate School of Life Science, Hokkaido University Faculty of Advanced Life Science, Hokkaido University

• Ijiro Kuniharu

  Research Institute for Electronic Science (RIES), Hokkaido University Global Station for Soft Matter, Global Institution for Collaborative Research and Education, Hokkaido University

• Yurimoto Hisayoshi

  Natural History Sciences, Hokkaido University

抄録

Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm. The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable isotope labeling. Key words: stable isotope, chromosome replication, semi-conservative replication, imaging, mass spectrometry

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 46 (2), 95-101, 2021

  日本細胞生物学会

キーワード

• stable isotope
• chromosome replication
• semi-conservative replication
• imaging
• mass spectrometry

詳細情報

• CRID: 1390008688858104960
• NII論文ID: 130008118990
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.21011
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 032032636
• PubMed: 34565768
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I032032636
• Web Site: https://www.jstage.jst.go.jp/article/csf/46/2/46_21011/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2023116185
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可