Ubiquitous Distribution of Azole-Resistant <i>Aspergillus fumigatus</i>- Related Species in Outdoor Environments in Japan

• Watanabe Kohei

  School of Pharmacy, Nihon University

• Yaguchi Takashi

  Medical Mycology Research Center, Chiba University

• Hirose Dai

  School of Pharmacy, Nihon University

Bibliographic Information

Other Title

• Ubiquitous Distribution of Azole-Resistant Aspergillus fumigatus-Related Species in Outdoor Environments in Japan

Abstract

Aspergillus fumigatus-related species are responsible for causing aspergillosis, which is a fatal infectious disease. Recently, there has been a series of reports of A. fumigatus-related species that are resistant to azole drugs used in clinical practice for the treatment of fungal infections. Some of these species have been isolated from outdoor environments. Testing the drug susceptibility of the strains from outdoor environments, therefore, is important. In this study, we isolated and cultured 72 strains of A. fumigatus-related species from the outdoor environment in Japan. The isolates identified via morphological observation and molecular phylogenetic analysis were Aspergillus felis, Aspergillus lentulus, Aspergillus pseudoviridinutans, Aspergillus udagawae, and Aspergillus wyomingensis. The results of the drug susceptibility testing revealed that A. felis (6 of 14 strains) and A. pseudoviridinutans (13 of 17 strains) were resistant to itraconazole (ITCZ), with 4 mg/L or higher minimum inhibitory concentrations (MICs). The voriconazole (VRCZ)-resistant strains with 4 mg/L or higher MICs were A. felis (14 of 14), A. lentulus (4 of 4), A. pseudoviridinutans (15 of 17), A. udagawae (23 of 34), A. wyomingensis (1 of 3), and A. pseudoviridinutans (1 of 3). Among them, A. felis (1 of 14) and A. pseudoviridinutans (7 of 17) demonstrated 8 mg/L or higher MICs for ITCZ and VRCZ. These results indicate that A. fumigatus-related species resistant to ITCZ and VRCZ are widely distributed in outdoor environments in Japan.

Journal

• Medical Mycology Journal

  Medical Mycology Journal 62 (4), 71-78, 2021

  The Japanese Society for Medical Mycology

Keywords

• <i>Aspergillus fumigatus</i>
• drug susceptibility
• <i>TUB-2</i>
• <i>Viridinutantes</i>
• voriconazole

Details

• CRID: 1390571704643936000
• NII Article ID: 130008122835
• NII Book ID: AA12518136
• DOI: 10.3314/mmj.21-00014
• ISSN: 2186165X
• ISSN: 21856486
• NDL BIB ID: 031817228
• Web Site: http://id.ndl.go.jp/bib/031817228
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031817228
• Web Site: https://www.jstage.jst.go.jp/article/mmj/62/4/62_21-00014/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022266817
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed