Electroporation-Based <i>ex Vivo</i> Gene Delivery into Dendritic Cells by Anionic Polymer-Coated Versatile Nuclear Localization Signal/pDNA Complex

• Kanazawa Takanori

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences School of Pharmaceutical Sciences, University of Shizuoka

• Hoashi Yuki

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Ibaraki Hisako

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Takashima Yuuki

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• Okada Hiroaki

  School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

Bibliographic Information

Other Title

• Electroporation-Based ex Vivo Gene Delivery into Dendritic Cells by Anionic Polymer-Coated Versatile Nuclear Localization Signal/pDNA Complex

Abstract

In this study, we focused on a nuclear localization signal (NLS)-based versatile peptide vector, designed by us, combined with electroporation (EP) to establish an efficient gene delivery system to non-dividing or slow growing dendritic cells. We determined the intranuclear transport, gene expression, and cell viability in JAWS II mouse dendritic cells transfected with the green fluorescent protein (GFP) expression plasmid DNA alone (naked pEGFP); positive charged complex of NLS derivative STR-CH2SV40H2C, and pEGFP (binary complex); or negative charged complex of the binary complex with a biocompatible polyanion, γ-polyglutamic acid (ternary complex) combined with or without EP application. Although the binary complex showed higher nuclear transport and GFP expression in the absence of EP than those for naked pEGFP, the combination of EP significantly decreased the cell viability and did not improve the efficiency of compared gene expression. However, in the ternary complex, the intranuclear transport and GFP expression efficiency were significantly higher than those of naked pEGFP and the binary complex when combined with EP, and there was no decrease in cell viability. The results suggest that polyanion-coated ternary complex with EP is useful for non-viral gene delivery system into non-dividing cells for ex vivo gene/cell therapy.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 44 (12), 1866-1871, 2021-12-01

  The Pharmaceutical Society of Japan

Keywords

• <i>ex vivo</i> gene therapy
• nuclear localization signal
• electroporation
• dendritic cell
• nuclear localization signal-based versatile peptide vector

Details

• CRID: 1390290239004477824
• NII Article ID: 130008123496
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b21-00559
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 031837962
• Web Site: http://id.ndl.go.jp/bib/031837962
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031837962
• Web Site: https://www.jstage.jst.go.jp/article/bpb/44/12/44_b21-00559/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2023019135
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed