Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells
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- ISHIGURE Hiroshi
- Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry
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- KAWAKI Harumi
- Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry
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- SHINTANI Kohei
- Department of Dental Materials Science, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry
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- UENO Kyohei
- Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry
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- MIZUNO-KAMIYA Masako
- Chemistry Laboratory, Department of Management and Information studies, Asahi University School of Business Administration
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- TAKAYAMA Eiji
- Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry
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- HOTTA Masato
- Asahi University
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- KONDOH Nobuo
- Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry
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- NIKAIDO Toru
- Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry
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Abstract
<p>Numerous studies have shown that the sustained release of ions from dental restorative materials have acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, the release behavior and bioresponsiveness of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types of media based on human dental pulp-derived stem cell (hDPSC) responses. The hDPSCs were cultured for 1–7 days in S-PRG eluates diluted with varying amounts of cell culture media. S-PRG released several types of ions, such as F−, Sr2+, Na+, Al3+, BO33−, and SiO32−. The balance of eluted ions differed depending on the dilution and solvent, which in turn affected the cytotoxicity, cell morphology, cell proliferation, and alkane phosphatase activity of hDPSCs, among other properties. The results suggest that tailored S-PRG filler eluates could be designed and prepared for application in dental practice.</p>
Journal
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- Dental Materials Journal
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Dental Materials Journal 40 (6), 1329-1337, 2021-11-25
The Japanese Society for Dental Materials and Devices