Exposure to (–)-Xanthatin during the Haploid Formation of Mouse Spermatocyte GC-2spd(ts) Cells, an <i>in vitro</i> Male Germ Cell Model

• Hirao-Suzuki Masayo

  Laboratory of Xenobiotic Metabolism and Environmental Toxicology, Faculty of Pharmaceutical Sciences, Hiroshima International University

• Takeda Shuso

  Laboratory of Molecular Life Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University

• Shindo Mitsuru

  Institute for Materials Chemistry and Engineering, Kyushu University

Abstract

We and others have previously reported the possible usefulness of (–)-xanthatin, one of the naturally occurring xanthanolides present in the Cocklebur plant, as an anticancer cytotoxic agent in both in vivo and in vitro settings. Anticancer agents generally act as a double-edged sword during cancer chemoprevention, as these drugs can cause infertility in males and females. Although (–)-xanthatin is known to be an anti-proliferator against cancer cells, the adverse effect on spermatogenesis has not yet been investigated, even in vitro. In this study, we utilized chemically synthesized pure (–)-xanthatin to analyze whether this molecule affects the appearance of haploid cells in the population of a GC-2spd(ts) cell line, an in vitro model of male germ cells. We did not observe any remarkable effects on the haploid formation of GC-2spd(ts) at a (–)-xanthatin concentration below 0.5 μM.

Journal

• BPB Reports

  BPB Reports 4 (6), 202-205, 2021

  The Pharmaceutical Society of Japan

Keywords

• (–)-xanthatin
• haploid
• GC-2spd(ts) cells
• spermatocyte

Details

• CRID: 1390853423037366272
• NII Article ID: 130008131874
• DOI: 10.1248/bpbreports.4.6_202
• ISSN: 2434432X
• Web Site: https://www.jstage.jst.go.jp/article/bpbreports/4/6/4_202/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2023054815
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed