Simple and rapid RNA detection using cationic copolymer-chaperoned MNAzyme (ACEzyme)

• YOSHIDA Naoki

  Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

• HANPANICH Orakan

  Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

• HAYASHI Raito

  Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

• SHIMADA Naohiko

  Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

• MARUYAMA Atsushi

  Department of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

抄録

Simple, rapid, and accurate diagnosis is indispensable for broadercontrol measures, search for routes of infection, and understanding treatment status of emerging infectious diseases. Although PCR and LAMP are commonly used, in cases where the pathogen is an RNA virus, there are issues in terms of simplicity and speed, as multi-step operations including reverse transcription (RT) and many reagents, as well as time and expertise in optimizing primers and probes, are required. To solve these issues, we propose the artificial chaperone-enhanced MNAzyme (ACEzyme), which combines the functions of the multicomponent nucleic acid enzyme (MNAzyme) and cationic copolymers. PLL-g-Dex has artificial nucleic acid chaperone activity and can act as a molecular sensing system. ACEzyme resulted in an approximately 2,700-fold increase in MNAzyme activity. In this study, we showed that ACEzyme was also useful when working with RNA targets with robust high-order structures, and had high selectivity for single nucleotide mutation detection.

収録刊行物

• Translational and Regulatory Sciences

  Translational and Regulatory Sciences 3 (3), 102-105, 2021

  キャタリストユニット

キーワード

• multicomponent nucleic acid enzyme (MNAzyme)
• cationic copolymer
• simple RNA detection method
• mismatch discrimination
• isothermal detection

詳細情報

• CRID: 1390009018100982016
• NII論文ID: 130008133945
• DOI: 10.33611/trs.2021-026
• ISSN: 24344974
• Web Site: https://www.jstage.jst.go.jp/article/trs/3/3/3_2021-026/_pdf
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可