The L-type Amino Acid Transporter (LAT1) Expression in Patients with Scoliosis

• Demura Satoru

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Hinoi Eiichi

  Department of Bioactive Molecules, Gifu Pharmaceutical University United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University

• Kawakami Noriaki

  Department of Orthopaedic Surgery, Ichinomiya Nishi Hospital

• Handa Makoto

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Yokogawa Noriaki

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Hiraiwa Manami

  Department of Bioactive Molecules, Gifu Pharmaceutical University

• Kato Satoshi

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Shinmura Kazuya

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Shimizu Takaki

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Oku Norihiro

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Annen Ryohei

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Kobayashi Motoya

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Yamada Yohei

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Nagatani Satoshi

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

• Iezaki Takashi

  Department of Bioactive Molecules, Gifu Pharmaceutical University

• Taniguchi Yuki

  Department of Orthopaedic Surgery, The University of Tokyo

• Tsuchiya Hiroyuki

  Department of Orthopaedic Surgery Graduate School of Medical Sciences Kanazawa University

Abstract

Introduction: Amino acid transporters are transmembrane proteins that are known to mediate the transfer of amino acids. As one of the amino acid transporters, LAT1, which is encoded by Slc7a5, mediates the cellular uptake of the essential amino acids. Recently, most studies have focused on examining the relationship between LAT1 and skeletal formation in terms of development. However, little is known regarding the clinical features of LAT1 in the cartilage, which might result in the development of skeletal deformities such as scoliosis. Thus, the aim of this study was to investigate the expression of L-type amino acid transporter 1 (LAT1) and its solute carrier transporter 7a5 (Slc7a5) in patients with pediatric scoliosis and to compare with the relationship between LAT1 and Slc7a5 expression and their clinical features. Methods: We have prospectively recruited 56 patients who underwent corrective spinal fusion for scoliosis. The patients comprised 40 girls and 16 boys, with a mean age of 13.1 years at the time of surgery. There were 34 idiopathic scoliosis (IS) patients, whereas 22 were congenital scoliosis (CS) patients. During the surgery, an epiphyseal part of the spinous process at apical vertebra was harvested; then, LAT1 and Slc7a5 expressions in the cartilage were evaluated. Results: As per our findings, LAT1 expression was observed in the cartilage in 60.7% (34 out of 56) of the patients. LAT1 expression in IS patients was 76%, which were statistically higher compared to 36% in CS patients. When compared with LAT1 expression, no statistical difference was noted in terms of age, gender, body mass index (BMI), Cobb angle, and Risser grade. Meanwhile, the mean Slc7a5 expression in IS patients was determined to be significantly higher than that in CS patients. No significant correlation was observed between Slc7a5 expression and age, BMI, and Cobb angle. Conclusions: LAT1 and Slc7a5 expression in IS and CS patients showed significant differences. These expressions were found to be not correlated with age, stature, and severity of the deformity.

Journal

• Spine Surgery and Related Research

  Spine Surgery and Related Research 6 (4), 402-407, 2022-07-27

  The Japanese Society for Spine Surgery and Related Research

Keywords

• scoliosis
• L-type amino acid transporter 1
• solute carrier transporter 7a5
• idiopathic scoliosis
• congenital scoliosis

Details

• CRID: 1390011461950931328
• NII Article ID: 130008136374
• DOI: 10.22603/ssrr.2021-0189
• ISSN: 2432261X
• Web Site: https://www.jstage.jst.go.jp/article/ssrr/6/4/6_2021-0189/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2023170348
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed