TCTP interactomics in NF1-associated malignant tumor cells by affinity-purification and SWATH-MS

• Kobayashi Daiki

  Dept. Tumor Genetics & Biology, Graduate School of Medical Sciences, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan Department of Omics and Systems biology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

• Araki Norie

  Dept. Tumor Genetics & Biology, Graduate School of Medical Sciences, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan

抄録

Neurofibromatosis type 1 (NF1) is an autosomal dominant RASopathy disease that predisposes individuals to developing benign neurofibromas and malignant peripheral nerve sheath tumors (MPNST). The mechanisms of NF1-tumorigenesis or the radical curatives have not been established. Using a unique transcriptome and proteome integration method, iPEACH^1–4, we previously identified translationally controlled tumor protein (TCTP) as a novel biological target for NF1-associated tumors⁵. To evaluate the function of TCTP in NF1-associated malignant tumor cells, the cellular interactome of TCTP, overexpressed as Flag-tagged protein, was identified by affinity-purification using anti-FLAG antibody coupled with SWATH-MS analysis (AP-SWATH). The data described in this paper have been deposited to jPOST^6,7 with the identifier JPST000107.

収録刊行物

• Journal of Proteome Data and Methods

  Journal of Proteome Data and Methods 3 (0), 2-, 2021

  日本プロテオーム学会

キーワード

• NF1-associated tumors
• TCTP
• SWATH-MS

詳細情報

• CRID: 1390009294978352640
• NII論文ID: 130008140150
• DOI: 10.14889/jpdm.2021.0002
• ISSN: 24346454
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可