Artificially Created Reentry Circuit by Laser Irradiation Causes Atrial Tachycardia to Persist in Murine Atria

• Horii Shunpei

  Department of Cardiology, National Defense Medical College

• Yada Hirotaka

  Department of Cardiology, International University of Health and Welfare Mita Hospital

• Ito Kei

  Department of Cardiology, National Defense Medical College

• Tsujita Kazuhiro

  Department of Medical Engineering, National Defense Medical College

• Osaki Ayumu

  Department of Cardiology, National Defense Medical College

• Kagami Kazuki

  Department of Cardiology, National Defense Medical College

• Sato Atsushi

  Department of Cardiology, National Defense Medical College

• Kimura Toyokazu

  Department of Cardiology, National Defense Medical College

• Yasuda Risako

  Department of Cardiology, National Defense Medical College

• Toya Takumi

  Department of Cardiology, National Defense Medical College

• Namba Takayuki

  Department of Cardiology, National Defense Medical College

• Nagatomo Yuji

  Department of Cardiology, National Defense Medical College

• Ido Yasuo

  Department of Cardiology, National Defense Medical College

• Miyazaki Koji

  Department of Comprehensive Internal Medicine, Tokai University Hachioji Hospital

• Masaki Nobuyuki

  Department of Intensive Care Medicine, National Defense Medical College

• Ishihara Miya

  Department of Medical Engineering, National Defense Medical College

• Takase Bonpei

  Department of Intensive Care Medicine, National Defense Medical College

• Adachi Takeshi

  Department of Cardiology, National Defense Medical College

Abstract

Background:There is a gradual progression from paroxysmal to persistent atrial fibrillation (AF) in humans. To elucidate the mechanism involved, the creation of an artificial atrial substrate to persist AF in mice was attempted. Methods and Results:This study used wild type (WT) mice, but it is difficult to induce AF in them. A novel antegrade perfusion method from the left ventricle (LV) to enlarge both atria for artificial atrial modification was proposed in this study. Short duration AF was induced by burst pacing under this method. Optical mapping analysis revealed non-sustained focal type and meandering spiral reentrants after short duration AF. A tiny artificial substrate (~1.2 mm in diameter) was added in by laser irradiation to create a critical atrial arrhythmogenic substrate. Burst pacing was performed in a non-laser group (n=8), a circular-shape laser group (n=8), and a wedge-shaped dent laser group (n=8). We defined AF and atrial tachycardia (AT) as atrial arrhythmia (AA). Long-lasting AA was defined as lasting for ≥30 min. Long-lasting AA was observed in 0/8, 0/8, and 6/8 (75%) mice in each group. Optical mapping analysis revealed that the mechanism was AT with a stationary rotor around the irradiated margin. Conclusions:Regrettably, this study failed to reproduce persistent AF, but succeeded in creating an arrhythmic substrate that causes sustained AT in WT mice.

Journal

• Circulation Journal

  Circulation Journal 86 (2), 319-329, 2022-01-25

  The Japanese Circulation Society

Keywords

• Atrial fibrillation
• Artificial reentry
• Atrial tachycardia
• Laser irradiation

Details

• CRID: 1390009371654037504
• NII Article ID: 130008144263
• NII Book ID: AA11591968
• DOI: 10.1253/circj.cj-20-1286
• ISSN: 13474820
• ISSN: 13469843
• NDL BIB ID: 031948609
• PubMed: 34544960
• Web Site: http://id.ndl.go.jp/bib/031948609
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031948609
• Web Site: https://www.jstage.jst.go.jp/article/circj/86/2/86_CJ-20-1286/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2023044164
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed