Extracellular Vesicles Derived from 3T3-L1 Adipocytes Enhance Procoagulant Activity
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- Nakatani Eriko
- Department of Molecular Physiology and Pathology, Faculty of Pharma-Science, Teikyo University
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- Naito Yasuo
- Department of Molecular Physiology and Pathology, Faculty of Pharma-Science, Teikyo University
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- Ishibashi Kenichi
- Department of Molecular Physiology and Pathology, Faculty of Pharma-Science, Teikyo University
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- Ohkura Naoki
- Department of Molecular Physiology and Pathology, Faculty of Pharma-Science, Teikyo University
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- Atsumi Gen-ichi
- Department of Molecular Physiology and Pathology, Faculty of Pharma-Science, Teikyo University
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Abstract
<p>Obesity is associated with the risk of venous thromboembolism. Thrombi are constantly formed via the coagulation cascade and degraded by the fibrinolytic system, so they tend to form in obese individuals. Adipocytes are involved in thrombus formation in obesity, but it is not clear whether bioactive factors from adipocytes directly initiate or enhance coagulation and thrombosis. In this study, we confirmed that adipocyte-derived extracellular vesicles (ADEVs) enhance procoagulant activity in vitro. ADEVs prepared from the culture supernatant of mature 3T3-L1 adipocytes shortened plasma clotting times. Moreover, the effect of ADEVs on clotting time was weakened when using plasma lacking factors of the extrinsic pathway, but not the intrinsic pathway. ADEVs contain tissue factors and phosphatidylserine, which are involved in the extrinsic pathway, and blockade of these molecules diminished the effects of ADEVs on plasma clotting time. Additionally, the effect of ADEVs on plasma clotting time was further enhanced when cells were stimulated with the proinflammatory cytokine tumor necrosis factor-α. Thus, ADEVs may be a factor in thrombus formation in obesity.</p>
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 45 (2), 178-183, 2022-02-01
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390853879733579136
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- NII Article ID
- 130008150243
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- ISSN
- 13475215
- 09186158
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed