実験動物の画像情報に基づいた行動評価法の提案：撮像のフレームレートによる影響評価

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  • Quantification of the Behavior of the Experimental Animal from the Animal Movies：Studies on the Effects of the Image Acquisition Rate
  • ジッケン ドウブツ ノ ガゾウ ジョウホウ ニ モトズイタ コウドウ ヒョウカホウ ノ テイアン : サツゾウ ノ フレームレート ニ ヨル エイキョウ ヒョウカ

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<p>The purpose of this study is to propose a quantitative method of image analysis of the behavior of captured animals with video surveillance systems with a variable acquisition rate. A total of 11 male C57BL/6J mice (aged 6-119 weeks) were used for the experiments. The behavior of the animals in the house cage was recorded for two hours. Matlab was used for image analysis. The projected form of the animal was quantified in the binary image, and the center of gravity was measured to determine the position of the animal in the cage. In each animal position, an area of the animal form and the residence time of the center of gravity in each pixel were calculated. The amount of animal motion between two consecutive frames was also measured. To test the compression of the recorded movie, the image was resampled from 30 to 3 frames per second. If the acquisition rate was set at 3 frames per second, the animal’s travel distance and resting time was underestimated on average of 27±8% and 54±13%, respectively, relative to the captured image at 30 frames per second. In contrast, the maximum residence time was not influenced by the acquisition rate. Furthermore, referring to xy coordinate of the center of gravity of the animal, it was possible to compare the regional difference in the animal form. We observed a significantly larger area of the animal’s form in the surrounding areas relative to the center of the cage. The results were consistent with the short length of stay in the surrounding areas relative to the center of the cage, which indicate that the animal moved more actively near the wall of the cage. Finally, 24-hours monitoring of animal behavior with the infrared camera system at a rate of acquisition of 3 frames per second showed a successful characterization of the animal behavior patterns corresponding to the measurements of animal movements. In conclusion, the present method of image analysis based on the maximum residence time of the animal’s center of gravity allows for regional comparisons of animal behavior and for reliable quantification independent of the acquisition rate of the monitoring systems.</p>

収録刊行物

  • 生体医工学

    生体医工学 59 (6), 139-145, 2021-12-10

    公益社団法人 日本生体医工学会

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