Selective Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators

• Masataka Fukuyama

• Shinya Yamatogi

• Hao Ding

• Mizue Nishida

• Chika Kawamoto

• Yoshiteru Amemiya

• Takeshi Ikeda

• Tomohide Noda

• Seiji Kawamoto

• Kazuhisa Ono

• Akio Kuroda

• Shin Yokoyama

Abstract

Si ring optical resonators have been fabricated by the Si LSI technology using electron beam lithography and reactive ion etching. The resonator is applied for sensing receptor-analyte reaction using silicon-binding protein, Si-tag, which enables receptors immobilized on a Si dioxide surface with a controlled orientation. For the receptor, the major house-dust mite allergen called Der f 2 was used, and for the analyte anti-Der f 2 immunoglobulin type G (IgG), which is selectively bound to Der f 2, was used. Antiserum against Der f 2, which contains anti-Der f 2 IgG, was diluted to 1/100 with the pure water, and exposed to the resonator on which the Der f 2 was adsorbed with the Si-tag. Then the resonance wavelength was shifted by 0.18 nm, while exposure of the normal rabbit serum containing no Der f 2 IgG resulted in the peak shift less than half (0.07 nm). These results indicate the selective reaction between Der f 2 and anti-Der f 2 IgG.

Journal

• Japanese Journal of Applied Physics

  Japanese Journal of Applied Physics 49 (4S), 04DL09-, 2010-04-01

  IOP Publishing

Keywords

• General Physics and Astronomy
• General Engineering

Details

• CRID: 1360003446855389312
• NII Article ID: 210000068367
• DOI: 10.1143/jjap.49.04dl09
• ISSN: 13474065
• ISSN: 00214922
• ISSN: http://id.crossref.org/issn/13474065
• Web Site: https://iopscience.iop.org/article/10.1143/JJAP.49.04DL09
• Web Site: https://iopscience.iop.org/article/10.1143/JJAP.49.04DL09/pdf
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  • CiNii Articles