Optical observation of DNA motion during and immediately after nanopore translocation

• Hirohito Yamazaki

• Shintaro Ito

• Keiko Esashika

• Toshiharu Saiki

Abstract

Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.

Journal

• Applied Physics Express

  Applied Physics Express 9 (1), 017001-, 2015-12-03

  IOP Publishing

Keywords

• General Physics and Astronomy
• General Engineering

Details

• CRID: 1360284924861188608
• NII Article ID: 210000137762
• DOI: 10.7567/apex.9.017001
• ISSN: 18820786
• ISSN: 18820778
• Web Site: https://iopscience.iop.org/article/10.7567/APEX.9.017001
• Web Site: https://iopscience.iop.org/article/10.7567/APEX.9.017001/pdf
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