Radical reaction in aqueous media injected by atmospheric pressure plasma jet and protective effect of antioxidant reagents evaluated by single-molecule DNA measurement

• Hirofumi Kurita

• Mika Shimizu

• Kaori Sano

• Tomoko Nakajima

• Hachiro Yasuda

• Kazunori Takashima

• Akira Mizuno

Abstract

Nonthermal atmospheric pressure plasma (NTP) has been extensively studied for biological and medical applications in recent years. Chemically active species are generated and injected into aqueous media subjected to plasma exposure. They play an important role in the activation and/or inactivation of biomolecules, such as nucleic acids, lipid, and proteins, in aqueous media. To evaluate the effect of NTP on these biomolecules at the molecular level, we have been considering large DNA molecules to be used as a biomarker. The single-molecule-based analysis of strand breakages induced by NTP, namely, the atmospheric pressure plasma jet (APPJ), on large DNA molecules has been performed. We demonstrated the facile estimation of the rate of double-strand breaks induced by the APPJ. Using this analysis, we have evaluated both the intensity of radical reaction and the protective effect on the reaction by adding antioxidant agents.

Journal

• Japanese Journal of Applied Physics

  Japanese Journal of Applied Physics 53 (5S1), 05FR01-, 2014-04-04

  IOP Publishing

Keywords

• General Physics and Astronomy
• General Engineering

Details

• CRID: 1360284924866076544
• NII Article ID: 210000143887
• DOI: 10.7567/jjap.53.05fr01
• ISSN: 13474065
• ISSN: 00214922
• ISSN: http://id.crossref.org/issn/00214922
• Web Site: https://iopscience.iop.org/article/10.7567/JJAP.53.05FR01
• Web Site: https://iopscience.iop.org/article/10.7567/JJAP.53.05FR01/pdf
• Data Source:
  • Crossref
  • CiNii Articles
  • KAKEN