Protein crystallization with paper

• Miki Matsuoka

• Keisuke Kakinouchi

• Hiroaki Adachi

• Mihoko Maruyama

• Shigeru Sugiyama

• Satoshi Sano

• Hiroshi Y. Yoshikawa

• Yoshinori Takahashi

• Masashi Yoshimura

• Hiroyoshi Matsumura

• Satoshi Murakami

• Tsuyoshi Inoue

• Yusuke Mori

• Kazufumi Takano

Abstract

We developed a new protein crystallization method that incorporates paper. A small piece of paper, such as facial tissue or KimWipes, was added to a drop of protein solution in the traditional sitting drop vapor diffusion technique, and protein crystals grew by incorporating paper. By this method, we achieved the growth of protein crystals with reducing osmotic shock. Because the technique is very simple and the materials are easy to obtain, this method will come into wide use for protein crystallization. In the future, it could be applied to nanoliter-scale crystallization screening on a paper sheet such as in inkjet printing.

Journal

• Japanese Journal of Applied Physics

  Japanese Journal of Applied Physics 55 (5), 050302-, 2016-04-12

  IOP Publishing

Keywords

• General Physics and Astronomy
• General Engineering

Details

• CRID: 1360847874820624512
• NII Article ID: 210000146451
• DOI: 10.7567/jjap.55.050302
• ISSN: 13474065
• ISSN: 00214922
• ISSN: http://id.crossref.org/issn/13474065
• Web Site: http://stacks.iop.org/1347-4065/55/i=5/a=050302/pdf
• Web Site: http://stacks.iop.org/1347-4065/55/i=5/a=050302?key=crossref.36c19e0cd62d6559bd8533071158ee49
• Web Site: https://iopscience.iop.org/article/10.7567/JJAP.55.050302
• Web Site: https://iopscience.iop.org/article/10.7567/JJAP.55.050302/pdf
• Data Source:
  • Crossref
  • CiNii Articles
  • KAKEN