Development of micro‐inclined well array for trapping single cells

• Takuya Tsukamoto

  Gunma University 1‐5‐1, Tenjin‐cho Kiryu Gunma 376‐8515 Japan

• Naoki Furuya

  Kagawa University 2217‐20, Hayashi‐cho Takamatsu Kagawa 761‐0396 Japan

• Takuya Shimagami

  Kagawa University 2217‐20, Hayashi‐cho Takamatsu Kagawa 761‐0396 Japan

• Rui Sato

  Gunma University 1‐5‐1, Tenjin‐cho Kiryu Gunma 376‐8515 Japan

• Fusao Shimokawa

  Kagawa University 2217‐20, Hayashi‐cho Takamatsu Kagawa 761‐0396 Japan

• Kazuya Akimitsu

  Kagawa University 2217‐20, Hayashi‐cho Takamatsu Kagawa 761‐0396 Japan

• Takaaki Suzuki

  Gunma University 1‐5‐1, Tenjin‐cho Kiryu Gunma 376‐8515 Japan

Abstract

We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side‐wall were made using three‐dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells.

Journal

• Electronics and Communications in Japan

  Electronics and Communications in Japan 101 (10), 45-50, 2018-07-20

  Wiley

Keywords

• Applied Mathematics
• Electrical and Electronic Engineering
• Computer Networks and Communications
• General Physics and Astronomy
• Signal Processing

Details

• CRID: 1360567180045922688
• NII Article ID: 210000167479
• DOI: 10.1002/ecj.12117
• ISSN: 19429541
• ISSN: 19429533
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fecj.12117
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1002/ecj.12117
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