Molecular Weight Control of Curdlan (β-1,3-Glucan Polysaccharide) Provides Unique Polynucleotide Binding Properties

• Koumoto Kazuya

  Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu

• Kobayashi Hideki

  Arakawa Chemical Industries, Ltd.

• Mizu Masami

  Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu

• Kimura Taro

  Fukuoka Industrial Technology Center, Biotechnology and Food Research Institute

• Sakurai Kazuo

  Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu

• Kunitake Toyoki

  Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu

• Shinkai Seiji

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University

書誌事項

タイトル別名

• Molecular Weight Control of Curdlan (.BETA.-1,3-Glucan Polysaccharide) Provides Unique Polynucleotide Binding Properties

抄録

Recently, we found that schizophyllan belonging to a β-1,3-glucan polysaccharide family forms macromolecular complexes with certain single-stranded polynucleotides. The specific complex formation is originated from the β-1,3-glucan skeleton in the main chain. In this paper, we have reported that curdlan, which is a β-1,3-glucan polysaccharide without a side chain glucose unit, shows unique poly(C) binding properties different from those of schizophyllan; in the experiments using curdlan samples with the controlled molecular weight, the complexation ability appeared only in a limited “M_w range window”. Stoichiometric analysis revealed that the “M_w range window” appears as a result of the competition between curdlan–curdlan self-association and curdlan–polynucleotide complexation. Furthermore, when the experiments were extended to other polynucleotides such as poly(A), poly(dA) and poly(dT), the “M_w range window” also appeared at their characteristic molecular weights. We believe, therefore, that curdlan can be applicable, in spite of its cheapness, as a precious functional material to gene technology.

収録刊行物

• Polymer Journal

  Polymer Journal 36 (5), 380-385, 2004

  The Society of Polymer Science, Japan

キーワード

• Polysaccharide–Polynucleotide Complex
• β-1,3-Glucan
• Polynucleotide Molecular Weight
• Binding Window
• Curdlan
• Schizophyllan

詳細情報

• CRID: 1390282681264421120
• NII論文ID: 130004430593
• NII論文ID: 30001923896
• DOI: 10.1295/polymj.36.380
• ISSN: 13490540
• ISSN: 00323896
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
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