Uncoupling heart cell specification and migration in the simple chordate<i>Ciona intestinalis</i>

DOI PDF 被引用文献2件
  • Brad Davidson
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, CA 94720, USA
  • Weiyang Shi
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, CA 94720, USA
  • Michael Levine
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, CA 94720, USA

抄録

<jats:p>The bHLH transcription factor Mesp has an essential but ambiguous role in early chordate heart development. Here, we employ the genetic and morphological simplicity of the basal chordate Ciona intestinalis to elucidate Mesp regulation and function. Characterization of a minimal cardiac enhancer for the Ciona Mesp gene demonstrated direct activation by the T-box transcription factor Tbx6c. The Mespenhancer was fused to GFP, permitting high-resolution visualization of heart cells as they migrate and divide. The enhancer was also used to drive targeted expression of an activator form of Mesp, which induces heart formation without migration. We discuss the implications of Tbx6-Mespinteractions for the evolution of cardiac mesoderm in invertebrates and vertebrates.</jats:p>

収録刊行物

  • Development

    Development 132 (21), 4811-4818, 2005-11-01

    The Company of Biologists

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