Reconstitution of an apicoplast‐localised electron transfer pathway involved in the isoprenoid biosynthesis of <i>Plasmodium falciparum</i>

• René C. Röhrich

• Nadine Englert

• Katrin Troschke

• Armin Reichenberg

• Martin Hintz

• Frank Seeber

• Emanuela Balconi

• Alessandro Aliverti

• Giuliana Zanetti

• Uwe Köhler

• Matthias Pfeiffer

• Ewald Beck

• Hassan Jomaa

• Jochen Wiesner

抄録

In the malaria parasite Plasmodium falciparum isoprenoid precursors are synthesised inside a plastid‐like organelle (apicoplast) by the mevalonate independent 1‐deoxy‐ d ‐xylulose‐5‐phosphate (DOXP) pathway. The last reaction step of the DOXP pathway is catalysed by the LytB enzyme which contains a [4Fe–4S] cluster. In this study, LytB of P. falciparum was shown to be catalytically active in the presence of an NADPH dependent electron transfer system comprising ferredoxin and ferredoxin‐NADP + reductase. LytB and ferredoxin were found to form a stable protein complex. These data suggest that the ferredoxin/ferredoxin‐NADP + reductase redox system serves as the physiological electron donor for LytB in the apicoplast of P. falciparum .

収録刊行物

• FEBS Letters

  FEBS Letters 579 (28), 6433-6438, 2005-11-02

  Wiley

キーワード

• Cell Biology
• Genetics
• Molecular Biology
• Biochemistry
• Structural Biology
• Biophysics

詳細情報

• CRID: 1364233270011599872
• NII論文ID: 30002589144
• DOI: 10.1016/j.febslet.2005.10.037
• ISSN: 18733468
• ISSN: 00145793
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2005.10.037
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2005.10.037
• データソース種別:
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