Rederivation of Mice by Means of In Vitro Fertilization and Embryo Transfer.
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- SUZUKI Hiroshi
- Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
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- YOROZU Keigo
- Chugai Pharmaceutical Co., Ltd.
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- WATANABE Toshihiko
- Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
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- NAKURA Masao
- Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
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- ADACHI Jiro
- Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
書誌事項
- タイトル別名
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- Rederivation of Mice by Means of In Vit
この論文をさがす
抄録
In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.<br>
収録刊行物
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- Experimental Animals
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Experimental Animals 45 (1), 33-38, 1996
公益社団法人 日本実験動物学会
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詳細情報 詳細情報について
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- CRID
- 1390001205041956096
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- NII論文ID
- 10007528231
- 30010196822
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- NII書誌ID
- AA11032321
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- COI
- 1:STN:280:DyaK283ltVansQ%3D%3D
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL書誌ID
- 3920588
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- PubMed
- 8689578
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可