Gap‐43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat

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<jats:title>Abstract</jats:title><jats:p>Retinal ganglion cells (RGCs) in rats were retrogradely labeled with the fluorescent tracer Fluorogold (FG) and subjected to GAP‐43 and c‐JUN immunocytochemistry to identify those RGSs that are capable of regenerating an axon. After optic nerve section (ONS) and simultaneous application of FG to the nerve stump (group 1 experiments), GAP‐43 immunoreactive RGCs (between 2 and 21 days after ONS) always represented a subfraction of both FG‐labeled (i.e., surviving) RGCs and RGCs exhibiting c‐JUN. GAP‐43 immunoreactive RGCs represented 22% of RGCs normally present in rat retinae and 25% of surviving RGCs at 5 days after ONS but were reduced to 2% and 1%, which is 6% and 5% of survivors at 14 and 21 days, respectively. In animals that received a peripheral nerve (PN) graft after ONS (group 2 experiments), RGCs with regenerating axons were identified by FG application to the graft at 14 and 21 days. When examined at 21 and 28 days, all FG‐labeled RGCs exhibited GAP‐43 immunoreactivity, and FG/GAP‐43‐labeled RGCs were 3% and 2% of those resent in normal rat retinae. In relation to surviving. RGCs GAP‐43 immunoreactive RGCs represented 10% at both time points. FG‐/GAP‐43 labeled RGCs also exhibited c‐JUN, but c‐JUN immunoreactive RGCs were at both time points at least twice as numerous a FG‐/GAP‐43‐labeled RGCs. These data suggest that regenerating axons in PN grafts derive specifically from GAP‐43 reexpressing RGCs. Appearance of GAP‐43 immunoreactivity may therefore identify those RGCs that are capable of axonal regeneration or sprouting. 1994 John Wiley & Sons, Inc.</jats:p>

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