Enhanced H⁺/ATP coupling ratio of H⁺‐ATPase and increased
14‐3‐3 protein content in plasma membrane of tomato cells upon osmotic shock

• Loubna Kerkeb

• Kees Venema

• Juan Pedro Donaire

• María Pilar Rodríguez‐Rosales

抄録

Modulation of proton extrusion and ATP‐dependent H + transport through the plasma membrane in relation to the presence of 14‐3‐3 proteins in this membrane in response to osmotic shock was studied in tomato ( Lycopersicon esculentum Mill. cv. Pera) cell cultures. In vivo H + extrusion by cells was activated rapidly and significantly after adding 100 m M NaCl, 100 m M KCl, 50 m M Na 2 SO 4 , 1.6% sorbitol or 2 µ M fusicoccin to the medium. The increase in H + extrusion by cells treated with 100 m M NaCl was correlated with an increase of H + transport by the plasma membrane H + ‐ATPase (EC 3.6.1.35), but not with changes in ATP hydrolytic activity of this enzyme, suggesting an increased coupling ratio of the enzyme. Immunoblot experiments showed increased amounts of 14‐3‐3 proteins in plasma membrane fractions isolated from tomato cells treated with 100 m M NaCl as compared to control cells without changing the amount of plasma membrane H + ‐ATPase. Together, these data indicate that in tomato cells an osmotic shock could enhance coupling between ATP hydrolysis and proton transport at the plasma membrane through the formation of a membrane 14‐3‐3/H + ‐ATPase complex.

収録刊行物

• Physiologia Plantarum

  Physiologia Plantarum 116 (1), 37-41, 2002-09

  Wiley

キーワード

• Cell Biology
• Plant Science
• Genetics
• General Medicine
• Physiology

詳細情報

• CRID: 1360011143592304512
• NII論文ID: 30013423149
• NII書誌ID: AA00774354
• DOI: 10.1034/j.1399-3054.2002.1160105.x
• ISSN: 13993054
• ISSN: 00319317
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1034%2Fj.1399-3054.2002.1160105.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1399-3054.2002.1160105.x
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