Toxicity and immunogenicity of <i>Aeromonas salmonicida</i> extracellular products to salmonids

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Abstract

<jats:p><jats:bold>Abstract. </jats:bold> <jats:italic>Aeromonas salmonicida</jats:italic> extracellular products (ECP) were fractionated by gel filtration chromatography. Three protein fractions were found. The first and second fractions showed low and high haemolytic activity, respectively, whereas the third fraction showed protcolytic activity. By intramuscular injection into juvenile rainbow trout, the median lethal doses of the first, second and third fractions were calculated at >669, 152 and <1514μg/g body weight, respectively. Cytopathic effects of the fractionated ECP to coho salmon lymphocytes were observed <jats:italic>in vitro.</jats:italic> The first and second fractions caused cell deformation, nuclear granulation and cytoplasmic streaming after 3h. No cytologic effects with the third fraction were observed. A mixture of the first and third fractions, a mixture of the second and third fractions, and non‐fractionated ECP caused nuclear granulation and cytoplasmic streaming after 3Qmin. Using immunodiffusion analysis, the first and second fractions formed a single precipitating line each against white‐spotted char anti‐ECP sera, but the third fraction did not formed a precipitating line against the antisera.</jats:p>

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