Synaptic scaffolding molecule interacts with Axin

• Susumu Hirabayashi

• Wataru Nishimura

• Junko Iida

• Ai Kansaku

• Shosei Kishida

• Akira Kikuchi

• Noriaki Tanaka

• Yutaka Hata

抄録

Synaptic scaffolding molecule (S‐SCAM) is a synaptic protein that consists of PDZ domains, a guanylate kinase domain, and WW domains. It interacts with N ‐methyl‐ d ‐aspartate receptor subunits, neuroligin, and β‐catenin. Here, we identified Axin as a novel binding partner of S‐SCAM. Axin was co‐immunoprecipitated with S‐SCAM from rat brain, detected in the post‐synaptic density fraction in rat brain subcellular fractionation, and partially co‐localized with S‐SCAM in neurons. The guanylate kinase domain of S‐SCAM directly bound to the GSK3β‐binding region of Axin. S‐SCAM formed a complex with β‐catenin and Axin, but competed with GSK3β for Axin‐binding. Thereby, S‐SCAM inhibited the Axin‐mediated phosphorylation of β‐catenin by GSK3β.

収録刊行物

• Journal of Neurochemistry

  Journal of Neurochemistry 90 (2), 332-339, 2004-06-07

  Wiley

キーワード

• Cellular and Molecular Neuroscience
• Biochemistry

詳細情報

• CRID: 1363670319871211392
• NII論文ID: 30014571901
• DOI: 10.1111/j.1471-4159.2004.02497.x
• ISSN: 14714159
• ISSN: 00223042
• ISSN: http://id.crossref.org/issn/00223042
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.2004.02497.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2004.02497.x
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  • CiNii Articles
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