Effect of Hypoglycemic Encephalopathy upon Amino Acids, High‐Energy Phosphates, and pH_i in the Rat Brain <i>In Vivo:</i> Detection by Sequential ¹H and ³¹P NMR Spectroscopy

• K. L. Behar

• J. A. den Hollander

• O. A. C. Petroff

• H. P. Hetherington

• J. W. Prichard

• R. G. Shulman

抄録

Abstract: Metabolic alterations in amino acids, high‐energy phosphates, and intracellular pH during and after insulin hypoglycemia in the rat brain was studied in vivo by 1 H and 31 P nuclear magnetic resonance (NMR) spectroscopy. Sequential accumulations of 1 H and 31 P spectra were obtained from a double‐tuned surface coil positioned over the exposed skull of a rat while the electroencephalogram was recorded continuously. The transition to EEG silence was accompanied by rapid declines in phosphocreatine, nucleoside triphosphate, and an increase in inorganic orthophosphate in 31 P spectra. In 1 H spectra acquired during the same time interval, the resonances of glutamate and glutamine decreased in intensity while a progressive increase in aspartate was observed. Following glucose administration, glutamate and aspartate returned to control levels (recovery half‐time, 8 min); recovery of glutamine was incomplete. An increase in lactate was detected in the 1 H spectrum during recovery but it was not associated with any change in the intracellular pH as assessed in the corresponding 31 P spectrum. Phosphocreatine returned to control levels following glucose administration, in contrast to nucleoside triphosphate and inorganic orthophosphate which recovered to only 80% and 200% of their control levels, respectively. These results show that the changes in cerebral amino acids and high‐energy phosphates detected by alternating the collection of 1 H and 31 P spectra allow for a detailed assessment of the metabolic response of the hypoglycemic brain in vivo.

収録刊行物

• Journal of Neurochemistry

  Journal of Neurochemistry 44 (4), 1045-1055, 1985-04

  Wiley

キーワード

• Cellular and Molecular Neuroscience
• Biochemistry

詳細情報

• CRID: 1360576122622478720
• NII論文ID: 30014580568
• DOI: 10.1111/j.1471-4159.1985.tb08723.x
• ISSN: 14714159
• ISSN: 00223042
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.1985.tb08723.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.1985.tb08723.x
• データソース種別:
  • Crossref
  • CiNii Articles